期刊
CURRENT MICROBIOLOGY
卷 70, 期 2, 页码 298-306出版社
SPRINGER
DOI: 10.1007/s00284-014-0717-2
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资金
- Natural Science Foundation of China [31200031]
- Fundamental Research Funds for the Central Universities [xjj2012125]
- Open Project Program of the State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201201]
- China Postdoctoral Science Foundation [2013M532055]
Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O2-center dot), and hydroxyl free radicals (OH center dot) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.
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