期刊
CURRENT MICROBIOLOGY
卷 68, 期 5, 页码 575-580出版社
SPRINGER
DOI: 10.1007/s00284-013-0509-0
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资金
- National Natural Science Foundation of China [31201910]
- National Basic Research Program of China [2012CB518804]
- Science and Technology Research Foundation of Henan Province Educational Committee [13A230261, 14A230003]
- Foundation for University Key Teacher by the Ministry of Education of Henan Province [2013GGJS-068]
- Nanjing Science and Technology Committee [201201026]
- Cooperative innovation fund of production-college-research of Jiangsu Province [BY2011114]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Autoinducer-2 (AI-2) is a universal signal molecule mediating intra- and interspecies communication among bacteria. AI-2 is a byproduct of the LuxS enzyme during the catabolism of S-adenosylhomocysteine and plays critical roles in regulating various behaviors of bacteria. In our previous study, the function of LuxS in AI-2 production was verified in Streptococcus suis (SS). Decreased levels of SS biofilm formation and host-cell adherence as well as the inability to produce AI-2 were observed in SS having a luxS mutant gene. In this study, exogenous addition of a low concentration of AI-2 synthesized in vitro was found to promote biofilm formation and host-cell adherence. However, higher concentrations of AI-2 inhibited SS biofilm formation and host-cell adherence. Real-time PCR results showed that the mRNA level of virulence factors of SS biofilm, gdh, cps2, sly, and mrp increased and ef, fbps, and gapdh decreased with increasing AI-2 concentrations. These findings demonstrated that AI-2 supplemented exogenously acted as a concentration-dependent signaling molecule to regulate SS biofilm formation, host-cell adherence, and transcription levels of many virulence genes.
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