期刊
CURRENT MICROBIOLOGY
卷 61, 期 5, 页码 422-428出版社
SPRINGER
DOI: 10.1007/s00284-010-9633-2
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资金
- National Natural Science Foundation of China [30970413, 30870346]
- Science and Technology Planning Project Foundation of Guangdong, China [2007B020500002-4, 2008A020100023]
- Science and Technology Planning Project Foundation of Guangzhou Agricultural Bureau [GZCQC0702FG06054]
- Foundation of Guangdong Academy of Sciences [SF200810]
Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.
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