期刊
CURRENT GENETICS
卷 57, 期 1, 页码 13-23出版社
SPRINGER
DOI: 10.1007/s00294-010-0322-2
关键词
ku70; Gene targeting; Trichoderma virens; Laccase; Sclerotia; Mycoparasitism
资金
- Scuola Normale Superiore di Pisa, Italy
- Italian Ministry of University and Research (MIUR)
- Austrian Science Fund [P20559, T390]
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
- Austrian Science Fund (FWF) [T390, P20559] Funding Source: Austrian Science Fund (FWF)
The aim of this study was to apply a generated Delta tku70 strain with increased homologous recombination efficiency from the mycoparasitic fungus Trichoderma virens for studying the involvement of laccases in the degradation of sclerotia of plant pathogenic fungi. Inactivation of the non-homologous end-joining pathway has become a successful tool in filamentous fungi to overcome poor targeting efficiencies for genetic engineering. Here, we applied this principle to the biocontrol fungus T. virens, strain I10, by deleting its tku70 gene. This strain was subsequently used to delete the laccase gene lcc1, which we found to be expressed after interaction of T. virens with sclerotia of the plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Lcc1 was strongly upregulated at early colonization of B. cinerea sclerotia and steadily induced during colonization of S. sclerotiorum sclerotia. The Delta tku70 Delta lcc1 mutant was altered in its ability to degrade the sclerotia of B. cinerea and S. sclerotiorum. Interestingly, while the decaying ability for B. cinerea sclerotia was significantly decreased, that to degrade S. sclerotiorum sclerotia was even enhanced, suggesting the operation of different mechanisms in the mycoparasitism of these two types of sclerotia by the laccase LCC1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据