期刊
CURRENT BIOLOGY
卷 23, 期 17, 页码 1670-1676出版社
CELL PRESS
DOI: 10.1016/j.cub.2013.06.060
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) [23227001]
- NC-CARP project of the Ministry of Education, Culture, Sports, Science and Technology in Japan
- Bio-oriented Technology Research Advancement Institution (BRAIN)
- Funding Program for Next Generation World-Leading Researchers of JSPS [GS025]
- [19060009]
- [2301 2020]
- Grants-in-Aid for Scientific Research [23227001, 25840111] Funding Source: KAKEN
Flowering plants in the reproductive stage are particularly vulnerable to ambient temperature fluctuations [1-6]. Nevertheless, they maintain seed production under certain levels of exposure to temperature change. The mechanisms underlying this temperature tolerance are largely unknown. Using an in vitro Arabidopsis pollen tube culture, we found that a synthetic CLV3/ESR-related peptide, CLE45, prolonged pollen tube growth. A subsequent screen of Arabidopsis mutants of leucine-rich repeat receptor-like kinase genes identified two candidate receptors for CLE45 peptide, STERILITY-REGULATING KINASE MEMBER1 (SKM1) and SKM2. The double loss-of-function mutant was insensitive to CLE45 peptide in terms of pollen tube growth in vitro. The SKM1 protein actually interacted with CLE45 peptide. CLE45was preferentially expressed in the stigma in the pistil at 22 degrees C, but upon temperature shift to 30 degrees C, its expression expanded to the transmitting tract, along which pollen tubes elongated. In contrast, both SKM1 and SKM2 were expressed in pollen. Disturbance of CLE45-SKM1/SKM2 signaling transduction by either RNAi suppression of CLE45 expression or introduction of a kinase-dead version of SKM1 into skm1 plants reduced seed production at 30 degrees C, but not at 22 degrees C. Taken together with the finding that CLE45 peptide application alleviated mitochondrial decay during the in vitro pollen tube culture, these results strongly suggest that the pollen-pistil interaction via the CLE45-SKM1/SKM2 signaling pathway sustains pollen performance under higher temperatures, leading to successful seed production.
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