期刊
CURRENT BIOLOGY
卷 23, 期 8, 页码 671-677出版社
CELL PRESS
DOI: 10.1016/j.cub.2013.02.059
关键词
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资金
- National Institutes of Health [DP10D003878]
- Weston Havens Foundation
- Medical Research Council, UK
- MRC [MR/K010174/1, MR/J012254/1] Funding Source: UKRI
- Medical Research Council [MR/K010174/1B, G0600719B, MR/K010174/1, MR/J012254/1] Funding Source: researchfish
Replacement of wild insect populations with genetically modified individuals unable to transmit disease provides a self-perpetuating method of disease prevention but requires a gene drive mechanism to spread these traits to high frequency [1-3]. Drive mechanisms requiring that transgenes exceed a threshold frequency in order to spread are attractive because they bring about local but not global replacement, and transgenes can be eliminated through dilution of the population with wild-type individuals [4-6]. These features are likely to be important in many social and regulatory contexts [7-10]. Here we describe the first creation of a synthetic threshold-dependent gene drive system, designated maternal-effect lethal underdominance (UDMEL), in which two maternally expressed toxins, located on separate chromosomes, are each linked with a zygotic antidote able to rescue maternal-effect lethality of the other toxin. We demonstrate threshold-dependent replacement in single- and two-locus configurations in Drosophila. Models suggest that transgene spread can often be limited to local environments. They also show that in a population in which single-locus UDMEL has been carried out, repeated release of wild-type males can result in population suppression, a novel method of genetic population manipulation.
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