期刊
CURRENT BIOLOGY
卷 21, 期 20, 页码 1695-1703出版社
CELL PRESS
DOI: 10.1016/j.cub.2011.08.056
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资金
- Cancer Center from the National Cancer Institute [CA21765]
- National Institutes of Health [GM68418]
- American Cancer Society [RSG-07-144-01-CCG]
- American Lebanese Syrian Association Charities
Background: The centromere (CEN) DNA-kinetochore complex is the specialized chromatin structure that mediates chromosome attachment to the spindle and is required for high-fidelity chromosome segregation. Although kinetochore function is conserved from budding yeast to humans, it was thought that transcription had no role in centromere function in budding yeast, in contrast to other eukaryotes including fission yeast. Results: We report here that transcription at the centromere facilitates centromere activity in the budding yeast Saccharomyces cerevisiae. We identified transcripts at CEN DNA and found that Cbf1, which is a transcription factor that binds to CEN DNA, is required for transcription at CEN DNA. Chromosome instability of cbfl Delta cells is suppressed by transcription driven from an artificial promoter. Furthermore, we have identified Ste12, which is a transcription factor, and Dig1, a Ste12 inhibitor, as a novel CEN-associated protein complex by an in vitro kinetochore assembly system. Dig1 represses Ste12-dependent transcription at the centromere. Conclusions: Our studies reveal that transcription at the centromere plays an important role in centromere function in budding yeast.
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