期刊
CURRENT BIOLOGY
卷 18, 期 18, 页码 1355-1364出版社
CELL PRESS
DOI: 10.1016/j.cub.2008.07.097
关键词
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资金
- NIH [R01 DK070184, R01 GM056264, P30 CA42014]
- Huntsman Cancer Institute/Foundation
- Department of Oncological Sciences
- Caenorhabditis Genetics Center
- OMRF Knockout Group
- [T32 HD007491]
Background: FoxA factors are critical regulators of embryonic development and postembryonic life, but little is know about the upstream pathways that modulate their activity [1]. C. elegans pha-4 encodes a FoxA transcription factor that is required to establish the foregut in embryos and to control growth and longevity afterbirth [2-5]. We previously identified the AAA+ ATPase homolog ruvb-1 as a potent suppressor of pha-4 mutations [6]. Results: Here we show that ruvb-1 is a component of the Target of Rapamycin (TOR) pathway in C. elegans (CeTOR). Both ruvb-1 and let-363/TOR control nucleolar size and promote localization of box C/D snoRNPs to nucleoli, suggesting a role in rRNA maturation. Inactivation of let-363/TOR or ruvb-1 suppresses the lethality associated with reduced pha-4 activity. The CeTOR pathway controls protein homeostasis and also contributes to adult longevity [7, 8]. We find that pha-4 is required to extend adult lifespan in response to reduced CeTOR signaling. Mutations in the predicted CeTOR target rsks-1/1S6 kinase or in ife-2/e/F4E also reduce protein biosynthesis and extend lifespan [9-11], but only rsks-1 mutations requirepha-4 for adult longevity. In addition, rsks-1, but not ife-2, can suppress the larval lethality associated with pha-4 loss-of-function mutations. Conclusions: The data suggest that pha-4 and the CeTOR pathway antagonize one another to regulate postembryonic development and adult longevity. We suggest a model in which nutrients promote TOR and S6 kinase signaling, which represses pha-4/FoxA, leading to a shorter lifespan. A similar regulatory hierarchy may function in other animals to modulate metabolism, longevity, or disease.
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