期刊
CURRENT ANALYTICAL CHEMISTRY
卷 4, 期 3, 页码 250-262出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/157341108784911415
关键词
electrochemistry; stripping; nucleic acids; DNA; RNA; peptide nucleic acid; oligonucleotides; nucleobases; nucleosides; nucleotides; nucleic acids-modified electrodes; DNA damage; DNA hybridization
资金
- ASCR GA [IAA4004402]
- Czech Republic [203/07/1195]
- MEYS CR [LC06 035]
- institutional research plans [AV0Z50040507, AV0Z50040702]
The ability of nucleic acids (NA) and their components to accumulate at electrode surfaces and electrochemical properties of these species are closely related. This review is devoted to electrochemical stripping techniques applied in NA studies. Cathodic or anodic stripping voltammetry have been used for a highly sensitive determination of nucleobases, nucleosides, nucleotides or acid-hydrolyzed NAs, based on formation of sparingly soluble complexes of the NA constituents with electrochemically generated mercury or copper(I) ions. DNAs, RNAs and their synthetic analogues, either unmodified or labeled with electroactive markers, have been analyzed by adsorptive stripping (AdS) techniques with mercury, mercury film, amalgam and carbon-based electrodes. Strong adsorption of NAs at the electrode surfaces has been utilized in adsorptive transfer stripping (AdTS) techniques. In AdTS, a NA-modified electrode is prepared by adsorptive accumulation of the NA at the electrode surface, followed by transfer into background electrolyte not containing any NA. NA-modified electrodes can be used as simple electrochemical NA sensors. Recent applications of AdS and AdTS in NA microanalysis, in detection of DNA damage as well as in studies of DNA hybridization or DNA-protein interactions are reviewed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据