期刊
JOURNAL OF NEUROCHEMISTRY
卷 133, 期 4, 页码 582-589出版社
WILEY
DOI: 10.1111/jnc.13024
关键词
deuterated PUFA; lipid peroxidation; oxidative stress; alpha-synuclein
资金
- Wellcome/MRC Parkinson's Disease Consortium grant
- Medical Research Council [MC_G1000735] Funding Source: researchfish
- MRC [MC_G1000735] Funding Source: UKRI
Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated alpha-synuclein (alpha-Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric alpha-Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co-cultures of neurons and astrocytes. We found that oligomeric but not monomeric alpha-Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre-incubation of cells with isotope-reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of oligomeric alpha-Syn on lipid peroxidation. Inhibition of lipid peroxidation with D-PUFAs further protected cells from cell death induced by oligomeric alpha-Syn. Thus, lipid peroxidation induced by misfolding of alpha-Syn may play an important role in the cellular mechanism of neuronal cell loss in Parkinson's disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据