Spontaneous assembly of DNA-amorphous calcium phosphate nanocomposite spheres for surface-mediated gene transfer

Submicrospheres composed of amorphous calcium phosphate (ACP) and DNA spontaneously assembled on a substrate via homogeneous calcium phosphate (CaP) nucleation followed by deposition in a highly supersaturated labile CaP solution. The nanocomposite layer of sphere assembly showed higher gene transfer efficiency than a continuous film nanocomposite layer previously fabricated via heterogeneous nucleation in a metastable supersaturated solution.