Solid Forms of Amlodipine Besylate Physicochemical, Structural, and Thermodynamic Characterization

Amlodipine besylate, a calcium channel antagonist widely used in the treatment of hypertension and coronary artery disease, has been found to exist in four solid forms dihydrate, monohydrate, dihydrate, and amorphous A comprehensive characterization of these forms is provided, based on single-crystal and powder X-ray diffraction, thermal spectroscopic, microscopic and solubility measurements The crystal structure of the dihydrate is reported at both 25 and - 150 degrees C The crystal lattices of both the dihydrate and the stable monohydrate collapse upon removal of water molecules to create melt, from which the anhydrate subsequently crystallizes Rapid cooling of the dehydration induced melt from any of the hydrates produces the amorphous form Spectroscopic analysis in conjunction with the crystal structure analysis shows differences in the hydrogen bond networks in the different solid forms The kinetic solubility rank order at 37 degrees C in water is found to be anhydrate > monohydrate > dihydrate In these conditions the dihydrate is found to be the most stable form and other forms undergo solvent-mediated transformation (SMT) to yield the dihydrate Consistent with the SMT results van t Hoff analysis indicates anhydrate and dihydrate to be the stable phase above and below 71 degrees C respectively