期刊
CRYOBIOLOGY
卷 66, 期 2, 页码 96-104出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2012.10.008
关键词
Membrane; Cryopreservation; Actin cytoskeleton; Freeze/thaw
资金
- University of Minnesota Doctoral Dissertation Fellowship
- University of Minnesota McKnight Land-Grant Professorship
- NSF Grant [CBET-0644784]
In an earlier paper [35], we examined the mutual interaction between the actin cytoskeleton and the cell membrane and explored the role this interaction plays during freeze/thaw. In this follow-up paper, we investigate the physical and chemical stresses induced by freeze/thaw and explore the different mechanisms of damage caused by these stresses. Our results showed that changes in cell volume during freeze/thaw and the unfrozen water content in the solution alter the cytoskeleton stiffness, and the available membrane material. Combined with unfavorable ice-membrane interactions and increasing membrane stiffness, increased de-structuring of the membrane (such as bleb and microvilli formation) synergistically act on the membrane-cytoskeleton system generating irreversible damage. (C) 2012 Elsevier Inc. All rights reserved.
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