期刊
CRYOBIOLOGY
卷 61, 期 3, 页码 345-351出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2010.10.161
关键词
Cell-assembly technique; Rapid prototyping; Adipose-derived stem cells (ADSCs); Cryopreservation; Organ manufacturing
资金
- National Natural Science Foundation of China/the Research Grants Council of Hong Kong (NSFC/RGC) [50731160625]
- National Natural Science Foundation of China [30970748, 30540060, 30440043]
- National High Tech 863 Grant [2009AA043801]
- Tekes Finnish Funding Agency for Technology and Innovation [40079/07]
- Beijing Municipal Science and Technology Commission [H060920050530]
A new cell cryopreservation strategy for cell-assembling constructs was proposed. With this strategy, different concentrations of dimethysulfoxide (DMSO) and dextran-40 were directly incorporated into the cell/gelatin/alginate systems, prototyped according to a predesigned structure, cryopreserved at -80 degrees C for 10 days and followed a thawing process at 17 degrees C. The rheological properties, bonding water contents and melting points of the gelatin/alginate hydrogel systems were changed with the addition of different amounts of DMSO. The microscopy analysis, (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrasodium bromide (MTT) and hematoxylin and eosin (HE) staining indicated that the cell numbers were progressively in a selected DMSO concentration range. With DMSO 5% (v/v) alone, the metabolic rate in the construct attained (81.3 +/- 5.7)%. A synergistic effect was achieved with the combination of the DMSO/gelatin/alginate and dextran-40/gelatin/alginate hydrogel systems. These results indicated that the inclusion of DMSO and dextran-40 in the hydrogel could effectively enhance the cell preservation effects. This cryopreservation strategy holds the ability to be widely used in organ manufacturing techniques. (C) 2010 Elsevier Inc. All rights reserved.
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