期刊
CROP PROTECTION
卷 43, 期 -, 页码 213-215出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2012.08.008
关键词
Bacterial spot; Tomato; Pepper; atpD; Xanthomonads
类别
资金
- Grant agency of University of South Bohemia (GA JU) [064/2010/Z, 011/2011/Z]
- Grant Agency of the Czech Republic (GA CR) [521/08/H042]
- Ministry of Agriculture of the Czech Republic [QH71229]
- Grant Agency of the Academy of Sciences of the Czech Republic [AV0Z50510513]
This study presents the development of a PCR method for specific detection of Xanthomonas vesicatoria, causal agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed based on partial DNA sequence of the catalytic subunit of the ATP synthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria. (C) 2012 Elsevier Ltd. All rights reserved.
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