期刊
JOURNAL OF NATURAL PRODUCTS
卷 78, 期 12, 页码 3031-3040出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jnatprod.5b00833
关键词
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资金
- Australian Government under the Co-operative Research Centres Program-The Wound Management Innovation CRC Grant [2.12]
- University of South Australia
The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity of a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from the Australian medicinal plant Eremophila neglecta. Biofilm breakup activity of compound 1 on established Staphylococcus epidermidis and Staphylococcus aureus biofilms was compared to the antiseptic chlorhexidine and antibiotic levofloxacin. In a time course study, 1 was deposited onto polypropylene mesh to mimic a wound dressing and tested for biofilm removal. The ex-vivo cytotoxicity and effect on lipopolysaccharide-induced pro-inflammatory cytokine release were studied in mouse primary bone-marrow-derived macrophage (BMDM) cells. Compound 1 was effective in dispersing 12 h pre-established biofilms with a 7 log(10) reduction of viable bacterial cell counts, but was less active against 24 h biofilms (approximately 2 log(10) reduction). Compound-loaded mesh showed dosage-dependent biofilm-removing capability. In addition, compound 1 displayed a significant inhibitory effect on tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) secretion from BMDM cells, but interleukin-1 beta (IL-1 beta) secretion was not significant. The compound was not cytotoxic to BMDM cells at concentrations effective in removing biofilm and lowering cytokine release. These findings highlight the potential of this serrulatane diterpenoid to be further developed for applications in wound management.
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