期刊
CRITICAL REVIEWS IN BIOTECHNOLOGY
卷 30, 期 4, 页码 302-309出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/07388551.2010.490938
关键词
Biofuel; biomass; bioconversion; cellulase; cellulase assays
资金
- Ontario Graduate Scholarship
- NSERC
- NSERC-RCD
- NOHFC
- Ontario Research Chair
Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by beta-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and beta-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel approaches recently used for enzyme assays are summarized.
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