期刊
CRITICAL CARE CLINICS
卷 25, 期 2, 页码 357-+出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.ccc.2008.12.010
关键词
Blood substitute; Hemoglobin; Myoglobin; Nitric oxide; Oxygen carrier; Transfusion
资金
- National Institute of Neurological Disorders and Stroke [NS-38684]
- Johns Hopkins University School of Medicine
Molecular biology has been applied to the development of hemoglobin-based oxygen carrier (HBOC) proteins that can be expressed in bacteria or yeast. The transformation of the hemoglobin molecule into an HBOC requires a variety of modifications for rendering the acellular molecule of hemoglobin physiologically acceptable when transfused in circulation. Hemoglobins with different oxygen affinities can be obtained by introducing mutations at the heme pocket, the site of oxygen binding, or by introducing surface mutations that stabilize the hemoglobin molecule in the low-oxygen-affinity state. Modification of the size of the heme pocket is also used to hinder nitric oxide depletion and associated vasoconstriction. Introduction of cysteine residues on the hemoglobin surface allows formation of intermolecular bonds and formation of polymeric HBOCs. These polymers of recombinant hemoglobin have the characteristics of molecular size, molecular stability, and oxygen delivery to hypoxic tissue suitable for an HBOC.
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