期刊
CORAL REEFS
卷 30, 期 2, 页码 329-333出版社
SPRINGER
DOI: 10.1007/s00338-010-0687-1
关键词
Preservation methods; Real-time quantitative PCR; qPCR; Scleractinian coral; Storage solutions
资金
- NSF [06-23678]
- Hawai'i Invasive Species Council [53780/53781]
- Hawai'i Coral Reef Initiative
- NMSP MOA [2005-008/66882]
- University of Hawai'i [R/HE-1]
- NOAA Office of Sea Grant, Department of Commerce [NA09OAR4170060]
- NOAA [UNIHI-SEAGRANT-JC-09-30]
Specimen collection is time consuming and expensive, yet few laboratories test preservation methods before setting out on field expeditions. The most common preservation buffer used for coral specimens is > 70% EtOH. However, alternatives exist that are less flammable, easier to ship, and are widely used in other taxa. Here, we compare the effects of salt-saturated DMSO (SSD) and EtOH preservation buffers on post-extraction DNA quantity and quality. We found that soft tissue integrity was better maintained and higher quantities of DNA were extracted from EtOH-preserved specimens; however, by all other measures, SSD was a superior preservative to EtOH. Extractions of SSD-preserved specimens resulted in higher molecular weight DNA, higher PCR success, and more efficient amplification than specimens preserved in EtOH. Our results show that SSD is generally a superior preservative to EtOH for specimens destined for PCR studies, but species-specific differences indicate that preservation comparisons should be undertaken before collection and storage of samples.
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