期刊
CONTRAST MEDIA & MOLECULAR IMAGING
卷 6, 期 6, 页码 514-522出版社
WILEY-BLACKWELL
DOI: 10.1002/cmmi.456
关键词
cells; labels; tracking; iron oxide; MRI; Molday
资金
- Ontario Institute for Cancer Research
- Canadian Cancer Society Research Institute
Stem cells, cancer cells and immune cells were labeled by co-incubation with a new ultra-small iron oxide nanoparticle called Molday ION Rhodamine-B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and labeling efficiency. This study has shown that MIRB can be used to label both adherent and nonadherent cell lines, with high viability and loading levels sufficient for their detection in vivo by MRI at 3T. Copyright (C) 2011 John Wiley & Sons, Ltd.
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