期刊
JOURNAL OF MORPHOLOGY
卷 276, 期 10, 页码 1244-1257出版社
WILEY
DOI: 10.1002/jmor.20415
关键词
lizard; scales; corneous beta-proteins; ultrastructure
资金
- Comparative Histolab [RFO 2011]
The distribution of large corneous beta-proteins of 18-43 kDa (Ac37, 39, and 40) in the epidermis of the lizard Anolis carolinensis is unknown. This study analyses the localization of these beta-proteins in different body scales during regeneration. Western blot analysis indicates most protein bands at 40-50 kDa suggesting they mix with alpha-keratin of intermediate filament keratin proteins. Ac37 is present in mature alpha-layers of most scales and in beta-cells of the outer scale surface in some scales but is absent in the Oberhautchen, in the setae and beta-layer of adhesive pads and in mesos cells. In differentiating beta-keratinocytes Ac37 is present over 3-4 nm thick filaments located around the amorphous beta-packets and in alpha-cells, but is scarce in precorneous and corneous layers of the claw. Ac37 forms long filaments and, therefore, resembles alpha-keratins to which it probably associates. Ac39 is seen in the beta-layer of tail and digital scales, in beta-cells of regenerating scales but not in the Oberhautchen (and adhesive setae) or in beta- and alpha-layers of the other scales. Ac40 is present in the mature beta-layer of most scales and dewlap, in differentiating beta-cells of regenerating scales, but is absent in all the other epidermal layers. The large beta-proteins are accumulated among forming beta-packets of beta-cells and are packed in the beta-corneous material of mature beta-layer. Together alpha-keratins, large beta-proteins form the denser areas of mature beta-layer that may have a different consistence that the electron-paler areas. J. Morphol. 276:1244-1257, 2015. (c) 2015 Wiley Periodicals, Inc.
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