期刊
CONSERVATION GENETICS
卷 16, 期 3, 页码 513-522出版社
SPRINGER
DOI: 10.1007/s10592-014-0678-9
关键词
Biodiversity; Metabarcoding; Next-generation sequencing; Rare species; Type I error; Type II error
资金
- National Natural Science Foundation of China [31272665]
- One-Three-Five Program of the Research Center for Eco-Environmental Sciences [YSW2013B02]
- 100-Talent Program of the Chinese Academy of Sciences
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC Canadian Aquatic Invasive Species Network (CAISN)
- Canada Research Chair
Identification of rare species and mapping their distributions is crucial for understanding natural species distributions and causes and consequences of accelerating species declines. However, detection of rare species in both terrestrial and especially aquatic communities typically dominated by numerous microscopic species (i.e. rare biosphere) represents a formidable technical challenge. Rapid advances in high-throughput sequencing (HTS) technologies have revolutionized biodiversity studies in the rare biosphere, and also stimulated associated debates. Here we summarize research progress, discuss debates and problems, and propose possible solutions and future studies to address these issues. In addition, we provide take-home messages for experimental design and data interpretation when utilizing HTS techniques for rare biosphere exploration in ecology and conservation biology.
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