4.3 Article

Microsatellite markers for the giant kelp Macrocystis pyrifera

期刊

CONSERVATION GENETICS
卷 10, 期 6, 页码 1915-1917

出版社

SPRINGER
DOI: 10.1007/s10592-009-9853-9

关键词

Macrocystis pyrifera; Giant kelp; Microsatellites; Macroalga; Genetic diversity

资金

  1. US National Science Foundation [OCE96-14091, OCE99-82105, OCE06-20276]
  2. Fundacao para a Ciencia e Tecnologia [SFRH/BPD/14945/2004]
  3. MEGIKELP [PTDC/MAR/65461/2006]
  4. Fundação para a Ciência e a Tecnologia [SFRH/BPD/14945/2004] Funding Source: FCT

向作者/读者索取更多资源

We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.

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