期刊
CONSERVATION GENETICS
卷 10, 期 3, 页码 597-600出版社
SPRINGER
DOI: 10.1007/s10592-008-9586-1
关键词
Microsatellite loci; Rhinolophus pusillus; Genetic structure
资金
- Key Construction Program of the National 985'' Project
- Shanghai Priority Academic Discipline
- Royal Society Research Fellowship
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful for studying related species.
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