期刊
CONNECTIVE TISSUE RESEARCH
卷 55, 期 3, 页码 248-256出版社
INFORMA HEALTHCARE
DOI: 10.3109/03008207.2014.904856
关键词
Cardiac fibroblast; collagen; extracellular matrix; mechanical stretch; transforming growth factor beta
资金
- Health Research Board of Ireland [RP/2007/313]
- Health Research Board (HRB) [RP-2007-313] Funding Source: Health Research Board (HRB)
Understanding the impact of extracellular matrix sub-types and mechanical stretch on cardiac fibroblast activity is required to help unravel the pathophysiology of myocardial fibrotic diseases. Therefore, the purpose of this study was to investigate pro-fibrotic responses of primary human cardiac fibroblast cells exposed to different extracellular matrix components, including collagen sub-types I, III, IV, VI and laminin. The impact of mechanical cyclical stretch and treatment with transforming growth factor beta 1 (TGF beta 1) on collagen 1, collagen 3 and alpha smooth muscle actin mRNA expression on different matrices was assessed using quantitative real-time PCR. Our results revealed that all of the matrices studied not only affected the expression of pro-fibrotic genes in primary human cardiac fibroblast cells at rest but also affected their response to TGF beta 1. In addition, differential cellular responses to mechanical cyclical stretch were observed depending on the type of matrix the cells were adhered to. These findings may give insight into the impact of selective pathological deposition of extracellular matrix proteins within different disease states and how these could impact the fibrotic environment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据