期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 122, 期 -, 页码 163-169出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2015.09.006
关键词
Lipase; Miniemulsion; Immobilization; Ethanolysis; Ethyl esters
资金
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) [BEX 2569/14-0]
We report the synthesis of polyurethane (PU) with polyethylene glycol (PEG) by miniemulsion polymerization as technique to produce a support for enzyme immobilization and stabilization. In order to study the influence of molar weight and concentration of PEG in the enzyme-support interaction, different PEG (400, 4000 and 6000 Da) was used during the synthesis of PU-PEG support to immobilize Thermomyces lanuginosus lipase (TLL). The enzymatic derivatives were effectively used in the production of polyunsaturated fatty acids (PUFAs), particularly derivative TLL-PU-PEG6000. This derivative showed the best results in terms of K-m (0.183 mM) and V-max (45.79 mmol/min/mL), calculated by Lineweaver-Burk plot (double reciprocal plot) and catalytic efficient (250.22), 100% of yield immobilization and recovered activity, reaching the highest values to the ethyl esters production (260 mM ethyl esters. U-1) making this derivative a potential low cost catalyst for ethanolysis reactions in solvent free system. (C) 2015 Elsevier B.V. All rights reserved.
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