期刊
ANIMAL GENETICS
卷 46, 期 6, 页码 646-654出版社
WILEY
DOI: 10.1111/age.12330
关键词
abalone; heterosis; interspecific hybridization; matrix-assisted laser desorption; ionization time-of-flight; time-of-flight; two-dimensional gel electrophoresis
资金
- Hi-Tech Research and Development (863) Program of China [2012AA10A412]
- NSFC [U1205121, 31101896, 41106120, 40976093]
- Fujian Science and Technology Program [2012N5012]
- Earnmarked Fund for Modern Agro-Industry Technology Research System [CARS-48]
- National Sparking Plan Project [2011GA720001]
- Henan Science and Technology Program [14B240003]
- Henan Normal University [qd13053]
Proteomic analysis was performed on the eggs of hybrid abalone and their corresponding parental lines. A total of 915 +/- 19 stained protein spots were detected from Haliotis discus hannaifxH.discus hannaio (DD), 935 +/- 16 from H.giganteafxH.giganteao (GG) and 923 +/- 13 from H.giganteafxH.discus hannaio (GD). The spots from DD and GD were clustered together. The distance between DD and GG was maximal by hierarchical cluster analysis. A total of 112 protein gel spots were identified; of these, 59 were abalone proteins. The proteins were involved in major biological processes including energy metabolism, proliferation, apoptosis, signal transduction, immunity, lipid metabolism, electron carrier proteins, protein biosynthesis and decomposition, and cytoskeletal structure. Three of 20 differential expression protein spots involved in energy metabolism exhibited as upregulated in GD, 13 spots exhibited additivity, and four spots exhibited as downregulated in the offspring. Eleven protein spots were expressed at the highest level in DD. The proteins involved in stress responses included superoxide dismutase, peroxiredoxin 6, thioredoxin peroxidase and glutathione-S-transferase. Two of seven differential expression protein spots involved in response to stress exhibited as upregulated in GD, three exhibited additivity, and two exhibited as downregulated. These results might suggest that proteomic approaches are suitable for the analysis of hybrids and the functional prediction of abalone hybridization.
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