期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 89, 期 -, 页码 168-172出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.yjmcc.2015.10.034
关键词
Atherosclerosis; Macrophage; Shear stress
资金
- British Heart Foundation Centre of Research Excellence, Imperial College London
- European Commission under the Seventh Framework Programme (FP7) [201668]
- Kennedy Trustees
- British Heart Foundation [PG/15/49/31595, PG/09/090/28065, RG/11/13/29055] Funding Source: researchfish
Macrophages, a significant component of atherosclerotic plaques vulnerable to acute complications, can be pro-inflammatory (designated M1), regulatory (M2), lipid- (Mox) or Heme-induced (Mhem). We showed previously that low (LSS) and oscillatory (OSS) shear stress cause thin-cap fibroatheroma and stable smooth muscle cell-rich plaque formation respectively in ApoE-knockout (ApoE(-/-)) mice. Here we investigated whether different shear stress conditions relate to specific changes in macrophage polarization and plaque morphology by applying a shear stress-altering cast to the carotid arteries of high fat-fed ApoE(-/-) mice. The M1 markers iNOS and IRF5 were highly expressed in macrophage-rich areas of LSS lesions compared to OSS lesions 6 weeks after cast placement, while the M2 marker Arginase-1, and Mox/Mhem markers HO-1 and CD163 were elevated in OSS lesions. Our data indicates shear stress could be an important determinant of macrophage polarization in atherosclerosis, with low shear promoting M1 programming. (C) 2015 Elsevier Ltd. All rights reserved.
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