4.4 Article

Microfluidic gradient device for studying mesothelial cell migration and the effect of chronic carbon nanotube exposure

出版社

IOP PUBLISHING LTD
DOI: 10.1088/0960-1317/25/7/075010

关键词

microfluidics; static gradient device; cell migration; nanomaterial effects; human pleural mesothelial cells

资金

  1. National Science Foundation [NSF-1227359, NSF-1336205]
  2. WV EPSCoR program - National Science Foundation [EPS-1003907]
  3. Directorate For Engineering [1336205] Funding Source: National Science Foundation
  4. Div Of Chem, Bioeng, Env, & Transp Sys [1336205] Funding Source: National Science Foundation
  5. Div Of Chem, Bioeng, Env, & Transp Sys
  6. Directorate For Engineering [1227359] Funding Source: National Science Foundation

向作者/读者索取更多资源

Cell migration is one of the crucial steps in many physiological and pathological processes, including cancer development. Our recent studies have shown that carbon nanotubes (CNTs), similarly to asbestos, can induce accelerated cell growth and invasiveness that contribute to their mesothelioma pathogenicity. Malignant mesothelioma is a very aggressive tumor that develops from cells of the mesothelium, and is most commonly caused by exposure to asbestos. CNTs have a similar structure and mode of exposure to asbestos. This has raised a concern regarding the potential carcinogenicity of CNTs, especially in the pleural area which is a key target for asbestos-related diseases. In this paper, a static microfluidic gradient device was applied to study the migration of human pleural mesothelial cells which had been through a long-term exposure (4 months) to subcytotoxic concentration (0.02 mu g cm(-2)) of single-walled CNTs (SWCNTs). Multiple migration signatures of these cells were investigated using the microfluidic gradient device for the first time. During the migration study, we observed that cell morphologies changed from flattened shapes to spindle shapes prior to their migration after their sensing of the chemical gradient. The migration of chronically SWCNT-exposed mesothelial cells was evaluated under different fetal bovine serum (FBS) concentration gradients, and the migration speeds and number of migrating cells were extracted and compared. The results showed that chronically SWCNT-exposed mesothelial cells are more sensitive to the gradient compared to non-SWCNT-exposed cells. The method described here allows simultaneous detection of cell morphology and migration under chemical gradient conditions, and also allows for real-time monitoring of cell motility that resembles in vivo cell migration. This platform would be much needed for supporting the development of more physiologically relevant cell models for better assessment and characterization of the mesothelioma hazard posed by nanomaterials.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据