期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 25, 期 10, 页码 1583-1588出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1501.01039
关键词
Germ-free animals; germ-free mice; microbiota; gnotobiotic; isolator technology
The large intestine is a home for trillions of microbiota, which confer many benefits on the host, including production of vitamins, absorption of nutrients, pathogen displacement, and development of the immune system. For several decades, germ-free animals have been used to study the interaction between the host and its microbiota. This minireview describes the technical aspects for establishing and maintaining germ-free animals and highlights the advantages and disadvantages for germ-free animals as experimental models.
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