期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 112, 期 -, 页码 36-39出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2015.03.005
关键词
Xanthomonas arboricola pv. pruni; Diagnostic assays; Real-time PCR; LAMP
资金
- Instituto Nacional de Investigaciones Agrarias (INIA) [RTA2011-00140-C03-03]
- EU FP7 KBBE Project Q-Detect
- EU FP7 KBBE Project DROPSA
- European Science Foundation COST Action [873]
- COST Action [FA1104]
- EU FP7 ERA-NET EUPHRESCO II [ERA26-XAPDIAG-CITA]
- CYTED FRUT-SAN [112RT0441]
Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. (C) 2015 Elsevier B.V. All rights reserved.
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