High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species

We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agarwell diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000-5000 individual antimicrobial interactions. (C) 2015 Elsevier B.V. All rights reserved.