期刊
JOURNAL OF MEDICINAL FOOD
卷 18, 期 7, 页码 762-775出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/jmf.2014.3341
关键词
Inflammatory responses; BV-2 microglial cells; Nuclear factor-kappa B; Lonicera japonica THUNB; Lipopolysaccharide
资金
- Basic Science Research Program through National Research Foundation of Korea - Ministry of Education, Science and Technology, Republic of Korea [NRF-2012R1A5A2A28671860, NRF-2011-00503]
In the current study, we evaluated the anti-inflammatory effects of Lonicera japonica THUNB. (LJ) and its underlying molecular mechanism in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Our results indicated that LJ significantly inhibits LPS-stimulated production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)). In addition, LJ inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both the protein and mRNA levels. In LPS-stimulated BV-2 microglial cells, LJ inhibited proinflammatory cytokines and chemokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9) enzymatic activities, and/or mRNA expression, as well as reactive oxygen species (ROS) production. LJ significantly suppressed activation of nuclear factor-kappa B (NF-kappa B) and its translocation from the cytosol to the nucleus and suppressed the DNA-binding activity of NF-kappa B. Furthermore, LJ significantly inhibited phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase 1/2 (ERK 1/2), p38 mitogen-activated protein kinases (MAPKs), phosphatidylinositol 3-kinases (PI3K)/Akt, and Janus kinase 1 (JAK1)/signal transducer and activator of transcription (STAT)1/3. Collectively, our findings indicated that the antineuroinflammatory properties of LJ in LPS-induced BV-2 microglial cells is due to downregulation of proinflammatory cytokines and chemokines downstream of inhibition of NF-kappa B activation.
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