期刊
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
卷 31, 期 1, 页码 63-69出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cimid.2007.04.003
关键词
bacterial strain; growth phase; intestinal epithelium; multidrug resistance
资金
- NIDA NIH HHS [R01 DA-10200, R01 DA010200, R01 DA010200-10] Funding Source: Medline
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA010200] Funding Source: NIH RePORTER
Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimuriurn invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains. (C) 2007 Elsevier Ltd. All rights reserved.
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