期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 166, 期 -, 页码 126-133出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2014.07.009
关键词
Roundup (R); Genotoxicity; DNA damage recovery; Oxidative DNA damage repair; Base excision repair
资金
- Fundacao para a Ciencia e a Tecnologia (FCT
- Government of Portugal) [PTDC/AAC-AMB/114123/2009]
- FCT/MCTES [BI/CESAM/PTDC/AAC-AMB/114123/2009]
- European Regional Development Fund (ERDF) through COMPETE - Thematic Factors of Competitiveness Operational Program (POFC)
- Centre for Environmental and Marine Studies (CESAM)
- [SFRH/BPD/88947/2012]
- Fundação para a Ciência e a Tecnologia [PTDC/AAC-AMB/114123/2009] Funding Source: FCT
Roundup (R) is a glyphosate-based herbicide widely used with both agricultural and non-agricultural purposes, which has been demonstrated to represent a risk to non-target aquatic organisms, namely fish. Among the described effects to fish, genotoxicity has been pointed out as one of the most hazardous. However, the genotoxic mechanisms of Roundup (R) as well as the involvement of the oxidative DNA damage repair system are not entirely understood. Hence, this work aimed to improve the knowledge on the progression of DNA damage upon short-term exposure (3 days) and post-exposure (1-14 days) periods in association with DNA repair processes in Anguilla anguilla exposed to Roundup (R) (58 and 116 mu g L-1). DNA damage in hepatic cells was evaluated by the comet assay improved with the DNA-lesion specific endonucleases FPG and EndoIII. In order to evaluate the oxidative DNA damage repair ability, an in vitro base excision repair (BER) assay was performed, testing hepatic subcellular extracts. Besides the confirmation of the genotoxic potential of this herbicide, oxidative damage was implicit as an important mechanism of genetic damage, which showed to be transient, since DNA integrity returned to the control levels on the first day after cessation of exposure. An increased capacity to repair oxidative DNA damage emerging in the post-exposure period revealed to be a crucial pathway for the A. anguilla recovery; nevertheless, DNA repair machinery showed to be susceptible to inhibitory actions during the exposure period, disclosing another facet of the risk associated with the tested agrochemical. (C) 2014 Elsevier Inc. All rights reserved.
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