期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 155, 期 2, 页码 198-205出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2011.08.004
关键词
ABC transporter; Sea louse; Emamectin benzoate; SLICE (R); Drug resistance
资金
- Merck Animal Health
- Moredun foundation
- Scottish Salmon Producter's Organisation (SSPO)
- Marine Alliance for Science & Technology for Scotland (MASTS)
The salmon louse, Lepeophtheirus salmonis, is a crustacean ectoparasite of salmonid fish. At present, sea louse control on salmon farms relies heavily upon chemical treatments. Drug efflux transport, mediated by ABC transporters such as P-glycoprotein (Pgp), represents a major mechanism for drug resistance in parasites. We report here the molecular cloning of a new Pgp from the salmon louse, called SL-PGY1. A partial Pgp sequence was obtained by searching sea louse ESTs, and extended by rapid amplification of cDNA ends (RACE). The open reading frame of SL-PGY1 encodes a protein of 1438 amino acids that possesses typical structural traits of P-glycoproteins, and shows a high degree of sequence homology to invertebrate and vertebrate P-glycoproteins. In the absence of drug exposure. SL-PGY1 mRNA expression levels did not differ between a drug-susceptible strain of L salmonis and a strain showing a similar to 7-fold decrease in sensitivity against emamectin benzoate, the active component of the in-feed sea louse treatment SLICE (R) (Merck Animal Health). Aqueous exposure of the hyposensitive salmon louse strain to emamectin benzoate (24 h, 410 mu g/L) provoked a 2.9-fold upregulation of SL-PGY1. Adult male lice of both strains showed a greater abundance of SL-PGY1 mRNA than adult females. (C) 2011 Elsevier Inc. All rights reserved.
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