期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 155, 期 3, 页码 483-490出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2011.12.008
关键词
Cyprinus carpio L; Microcystin-LR; Reactive oxygen species; Oxidative stress; Protein phosphatase; HSP70
资金
- National Basic Research Program of China [2008CB418102]
Microcystin-LR (MC-LR) produced by cyanobacteria are potent specific hepatotoxins. So far the pathogenesis of environmental MC-LR toxicity to aquatic organisms has not been fully elucidated. In the present study the accumulation of MC-LR was investigated in various organs/tissues of Cyprinus carpio L (C. carpio) following exposure to MC-LR for 14 d at environmentally relevant concentrations (0.1 to 10 mu g L-1). Results showed that the presence of MC-LR enhanced toxin accumulation in all investigated organs and the highest accumulation was found in the liver of fish exposed to 5.0 mu g L-1 of MC-LR. An EPR analysis indicated center dot OH intensity in liver was significantly induced at 0.1 mu g L-1 of MC-LR and then restored when the MC-LR concentration was greater than 0.1 mu g L-1. After 14-day exposure, MC-LR (1.0-10.0 mu g L-1 of MC-LR) caused a pronounced promotion of glutathione S-transferase (GST) activity and a depletion of reduced glutathione (GSH) content in fish liver, which indicated that GSH was involved in detoxification of MC-LR and the conjugation reaction of MC-LR and GSH occurred. A mild oxidative damage was evidenced by the accumulation of malondialdehyde (MDA) level at 5.0 mu g L-1 of MC-LR exposure, but which was restored when the MC-LR concentration was increased to 10.0 mu g L-1. The responses of antioxidant enzymes and the induction of HSP70 expression might contribute to MC-LR tolerance of C. carpi. However, the protein phosphatase (PP) activities were strikingly inhibited in all treated groups. Thus, the overall toxicity of environmental MC-LR on C carpi seems to be initiated in the liver via both the ROS pathway and the PP inhibition pathway, and the latter might be more important when ambient MC-LR concentration is greater than 0.1 mu g L-1. More importantly, these results can help to support the evaluation on the potential effects of MC-LR under common environmental concentrations. (C) 2011 Elsevier Inc. All rights reserved.
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