期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 176, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2014.07.001
关键词
Nasutitermes corniger; Amylase; Cellulase; Serine proteases; Hemicellulase
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [303658/2009-6, 470197/2009-9, 305481/2012-6, 472546/2012-0]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Brazilian Ministry for Science, Technology and Innovation (MCTI) [01200.003711/2011-11]
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) [APQ-0653-2.08/10]
- FACEPE [IBPG-0459-2.08/11, APQ-0137-2.08/12, BCT-0421-2.08/12]
- CAPES
- CNPq
- Universidade Federal de Pernambuco
The digestive apparatus of termites may have several biotechnological applications, as well as being a target for pest control. This report discusses the detection of cellulases (endoglucanase, exoglucanase, and beta-glucosidase), hemicellulases (beta-xylosidase, alpha-L-arabinofuranosidase, and beta-D-xylanase), alpha-amylase, and proteases (trypsin-like, chymotrypsin-like, and keratinase-type) in gut extracts from Nasutitermes corniger workers and soldiers. Additionally, the effects of pH (3.0-11.0) and temperature (30-100 degrees dC) on enzyme activities were evaluated. All enzymes investigated were detected in the gut extracts of worker and soldier termites. Endoglucanase and p-xylanase were the main cellulase and hemicellulase, respectively. Zymography for proteases of worker extracts revealed polypeptides of 22, 30, and 43 kDa that hydrolyzed casein, and assays using protease inhibitors showed that serine proteases were the main proteases in worker and soldier guts. The determined enzyme activities and their response to different pH and temperature values revealed that workers and soldiers contained a distinct digestive apparatus. The ability of these termites to efficiently digest the main components of lignocellulosic materials stimulates the purification of gut enzymes. Further investigation into their biotechnological potential as well as whether the enzymes detected are produced by the termites or by their symbionts is needed. (C) 2014 Elsevier Inc. All rights reserved.
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