期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 166, 期 1, 页码 99-108出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2013.07.007
关键词
DNA methylation; zebrafish; development; vasa; rassf1
资金
- National Institute of Environmental Health Sciences [R01ES012710, R03ES018962, R21ES019940]
- South-Central Technology Transfer Award from Society of Toxicology
- Graduate Student Council Research Grant from the University of Mississippi [6402-21310-003-00]
- project of the Agricultural Research Service
DNA methylation is dynamic through the life of an organism. Previous studies have primarily focused on DNA methylation changes during very early embryogenesis. In this study, global and gene specific DNA methylation in zebrafish (Danio rerio) embryos, larvae and adult livers were compared. The percent methylation of cytosines was low in 2 to 4.3 h post fertilization (hpf) zebrafish embryos and was consistently higher in zebrafish older than 6 hpf. Furthermore, quantitative real-time PCR (qPCR) results showed relatively high DNA methyltransferase 1 (dnmt1) and low glycine N-methyltransferase (gnmt) mRNA expression in early embryogenesis. By studying methylation patterns and gene expression of five developmentally important genes, namely vase, Ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun and c-myca, we found that the timing of changes in DNA methylation patterns was gene specific, and changes in gene expression were not necessarily correlated with the DNA methylation patterns. (C) 2013 Elsevier Inc. All rights reserved.
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