期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 160, 期 1, 页码 32-39出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2011.05.005
关键词
Artemia franciscana; Late embryogenesis abundant; Hydrophilic protein; Post-diapause embryos; Hypersaline stress
资金
- National Natural Science Foundation of China [30221004]
- Ministry of Science and Technology of the People's Republic of China [2008ZX10004-013]
From post-diapause cysts of Artemia franciscana, we defined fourteen LEA (late embryogenesis abundant) and LEA-like genes, including four novel members (Afrlea 1-5, Afrlea3-5, Afrlea3-like1 and Afrlea3-like2), which were classified into four groups: G1, G3. G3-like (LEA group3-like), and 5MP-like (seed-maturation-protein-like), based on their conserved and diversified sequence motifs and amino acid compositions among bacteria, plants, and animals. We also validated six representative genes based on quantitative RT-PCR, including three LEA and two LEA3-like genes that are down-regulated when dehydrated cysts hatch to desiccation-intolerant larvae as well as one SMP-like gene that is slightly up-regulated. We further tested their responses to hypersaline stress for four representatives one from each group-and found that the expression of Afrleal-5 and Afrlea3-2 were inducible but not Afrlea3-like1 and Afrsmp-like. This result suggested that the LEA and LEA-like genes may play different roles in resistance to hypersaline stress. (C) 2011 Elsevier Inc. All rights reserved.
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