期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 154, 期 3, 页码 255-263出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2009.06.010
关键词
Biosynthesis; Cis-acting sequences; Fatty acyl Delta 6 desaturase; Gene expression; Long-chain polyunsaturated fatty acids; Promoter
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/C51237X/1]
- Biotechnology and Biological Sciences Research Council [BB/C51237X/1] Funding Source: researchfish
Fish vary in the ability to biosynthesise n-3 long-chain polyunsaturated fatty acids (LC-PUFA), with marine fish such as cod being inefficient in comparison to freshwater and salmonid fish. We investigated differences in the gene promoters of Delta 6 fatty acyl desaturase (Delta 6 FAD), a critical enzyme in LC-PUFA biosynthesis, in cod and salmon. Progressive deletions and targeted mutations of the promoters were tested for activity in a transfected Atlantic salmon (AS) cell line under low or high LC-PUFA treatment, and regions sufficient to direct transcription were identified. Comparison of these regions with sequences of corresponding regions of Delta 6 FAD genes from mammals, amphibians and fish indicated a remarkable conservation of binding sites for SREBPs and NF-Y. In addition to these sites, a site was identified in salmon with similarity to that recognised by Sp1 transcription factor, and which was required for full expression of the salmon Delta 6 FAD gene. The cod promoter was less active and lacked the Sp1 site. Eicosapentaenoic acid suppressed LC-PUFA synthesis in AS cells and also suppressed activity of the salmon Delta 6 FAD promoter although this activity was likely mediated through sites other than Sp1, possibly similar to those recognised by NF-Y and SREBP transcription factors. (C) 2009 Elsevier Inc. All rights reserved.
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