期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 117, 期 -, 页码 174-184出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2014.02.023
关键词
Interfacial rheology; Biofilms at liquid-liquid interfaces; Bacteria adsorption; Pendant drop tensiometry; Hydrophobicity of bacteria; BATH test; Pseudomonas putida KT2442
资金
- ETH Zurich [TH 32-1]
- Laboratory of Biotechnology at ETH Zurich
Bacterial adsorption to interfaces is a key factor in biofilm formation. One major limitation to understanding biofilm formation and development is the accurate measurement of bacterial cell adhesion to hydrophobic interfaces. With this study, bacterial attachment and biofilm growth over time at water-oil interface was monitored through interfacial rheology and tensiometry. Five model bacteria (Pseudomonas putida ICT2442, Pseudomonas putida W2, Salmonella typhimurium, Escherichia coli, and Bacillus subtilis) were allowed to adsorb at the water-oil interface either in their non-growing or growing state. We found that we were able to observe the initial kinetics of bacterial attachment and the transient biofilm formation at the water-oil interface through interfacial rheology and tensiometiy. Electrophoretic mobility measurements and bacterial adhesion to hydrocarbons (BATH) tests were performed to characterize the selected bacteria. To validate interfacial rheology and tensiometry measurements, we monitored biofilm formation utilizing both confocal laser scanning microscopy and light microscopy. Using this combination of techniques, we were able to observe the elasticity and tension development over time, from the first bacterial attachment up to biofilm formation. (C) 2014 Elsevier B.V. All rights reserved.
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