期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 122, 期 -, 页码 79-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.06.049
关键词
Silk fibroin film; Surface pattern; Endothelial cell; Cell morphology; Cell proliferation
资金
- National Natural Science Foundation of China [91027040, 21074084, 21374070, 21374069, 21204058]
- National Basic Research Program of China [2012CB821500]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
- Project of Scientific and Technologic Infrastructure of Suzhou [SZS201207]
Silk fibroin is an ideal blood vessel substitute due to its advantageous qualities including variable size, good suture retention, low thrombogenicity, non-toxicity, non-immunogenicity, biocompatibility, and controllable biodegradation. In this study, silk fibroin films with a variety of surface patterns (e.g. square wells, round wells plus square pillars, square pillars, and gratings) were prepared for in vitro characterization of human umbilical vein endothelial cell's (HUVEC) response. The affects of biomimetic length-scale topographic cues on the cell orientation/elongation, proliferation, and cell-substrate interactions have been investigated. The density of cells is significantly decreased in response to the grating patterns (70 +/- 3 nm depth, 600 +/- 8 nm pitch) and the square pillars (333 +/- 42 nm gap). Most notably, we observed the contact guidance response of filopodia of cells cultured on the surface of round wells plus square pillars. Overall, our data demonstrates that the patterned silk fibroin films have an impact on the behaviors of human umbilical vein endothelial cells. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据