期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 114, 期 -, 页码 164-169出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.10.006
关键词
Reduced graphene oxide; Silver nanoparticles; Direct electron transfer; Glucose oxidase; Glucose biosensor
资金
- National Science Council
- Ministry of Education of Taiwan (Republic of China)
The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E degrees) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (K-s) of GOx at the nanocomposite was calculated to be 5.27 s(-1), revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5 mM with a detection limit of 0.16 mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose. (C) 2013 Elsevier B.V. All rights reserved.
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