期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 119, 期 -, 页码 106-114出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.04.027
关键词
Superparamagnetic iron oxide; nanoparticles; Coating; Stability; T-2 relaxation; Toxicity; Particle internalization
资金
- College of Engineering Distinguished Faculty Fellowship
- NIH [HL 072900, 5P50HL083801]
- NSF [0910908]
- NIH/NIGMS [T32 GM008764]
- NIH/NIAID [T32 AI089673]
- NIH/NHLBI [HL072900-S, T32 HL007224]
Superparamagnetic iron oxide nanoparticles (SPIONs) are currently unavailable as MRI contrast agents for detecting atherosclerosis in the clinical setting because of either low signal enhancement or safety concerns. Therefore, a new generation of SPIONs with increased circulation time, enhanced image contrast, and less cytotoxicity is essential. In this study, monodisperse SPIONs were synthesized and coated with polyethylene glycol (PEG) of varying molecular weights. The resulting PEGylated SPIONs were characterized, and their interactions with vascular smooth muscle cells (VSMCs) were examined. SPIONs were tested at different concentrations (100 and 500 ppm Fe) for stability, T-2 contrast, cytotoxicity, and cellular uptake to determine an optimal formulation for in vivo use. We found that at 100 ppm Fe, the PEG 2K SPIONs showed adequate stability and magnetic contrast, and exhibited the least cytotoxicity and nonspecific cellular uptake. An increase in cell viability was observed when the SPION-treated cells were washed with PBS after 1 h incubation compared to 5 and 24h incubation without washing. Our investigation provides insight into the potential safe application of SPIONs in the clinic. (C) 2014 Elsevier B.V. All rights reserved.
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