Ultrasensitive IL-6 electrochemical immunosensor based on Au nanoparticles-graphene-silica biointerface

An Interleukin-6 (IL-6) electrochemical immunosensor was fabricated based on the Au nanoparticles (AuNP)-graphene-silica sol-gel as immobilization biointerface and AuNP-polydopamine (PDA)@carbon nanotubes (CNT) as the label of HRP-bound antibodies. The AuNP-graphene-silica sot-gel film was prepared in situ and modified on the ITO electrode, providing a stable network for the immobilization of antibody and exhibiting a dynamic working range of 1-40 pg/mL with a low detection limit of 0.3 pg/mL IL-6 (at 3 s). The results of serum samples with the sensor received an acceptable agreement with the ELISA method. Importantly, this method provided a promising ultrasensitive assay strategy for clinical applications. (C) 2013 Elsevier B.V. All rights reserved.