期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 115, 期 -, 页码 79-85出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.11.024
关键词
Polyethylenimine; Pyridoxal hydrochloride; Transfection efficiency; Cytotoxicity; Buffering capacity
资金
- CSIR [BSC0112]
Recently, polyethylenimines (PEIs) have emerged as efficient vectors for nucleic acids delivery. However, inherent cytotoxicity has limited their in vivo applications. To address this concern as well as to incorporate hydrophobic domains for improving interactions with the lipid bilayers in the cell membranes, we have tethered varying amounts of amphiphilic pyridoxyl moieties onto bPEI to generate a small series of pyridoxyl-PEI (PyP) polymers. Spectroscopic characterization confirms the formation of PyP polymers, which subsequently form stable complexes with pDNA in nanometric range with positive surface charge. The projected modification not only accounts for a decrease in the density of 1 degrees amines but also allows formation of relatively loose complexes with pDNA (et bPEI). Alleviation of the cytotoxicity, efficient interaction with cell membranes and easy disassembly of the pDNA complexes have led to the remarkable enhancement in the transfection efficiency of PyP/pDNA complexes in mammalian cells with one of the formulations, PyP-3/pDNA complex, showing transfection in similar to 68% cells compared to similar to 16% cells by Lipofectamine/pDNA complex. Further, the efficacy of PyP-3 vector has been established by delivering GFP-specific siRNA resulting in similar to 88% suppression of the target gene expression. These results demonstrate the efficacy of the projected carriers that can be used in future gene therapy applications. (C) 2013 Elsevier B.V. All rights reserved.
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