期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 102, 期 -, 页码 202-209出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2012.08.008
关键词
Biosurfactants; Candida sphaerica; Industrial waste; Oil; Toxicity
资金
- Foundation for the Support of Science and Technology of the State of Pernambuco (FACEPE)
- Coordination for the Improvement of Higher Level Education Personnel (CAPES)
- Research and Development Program from National Agency of Electrical Energy (ANEEL)
- Thermoelectric of Pernambuco (TERMOPE)
- National Council for Scientific and Technological Development (CNPq)
The development of less toxic, biodegradable, surfactants, such as biosurfactants, is a key strategy for acquiring environmentally friendly compounds. The aim of the present study was to employ an optimised medium containing 9% ground nut oil refinery residue and 9% corn steep liquor for the production of a biosurfactant by Candida sphaerica. Fermentation was carried out at 28 degrees C and 200 rpm for 144 h. Biosurfactant yield was 9 g/l. The biosurfactant reduced the surface tension of the medium to 25 mN/m, with a critical micelle concentration of 0.025%. The product demonstrated stability with regard to surface tension reduction and emulsification in a range of temperatures (5-120 degrees C) and pH values (2-12) as well as tolerance to high concentrations of NaCl (2-10%). Hydrophobicity tests indicate two possible insoluble substrate uptake mechanisms: direct interfacial uptake and biosurfactant-mediated transfer (cell contact with emulsified or solubilised hydrocarbons). The biosurfactant was characterised as an anionic glycolipid consisting of 70% lipids and 15% carbohydrates and demonstrated no toxicity to the microcrustacean Artemia salina or the vegetables Brassica oleracea, Solanum gilo, Lactuca sativa L. and Brassica oleracea L. The biosurfactant recovered 95% of motor oil adsorbed to a sand sample, demonstrating considerable potential for use in bioremediation processes, especially in the petroleum industry. (c) 2012 Elsevier B.V. All rights reserved.
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