Gold nanoparticles from Pro and eukaryotic photosynthetic microorganisms-Comparative studies on synthesis and its application on biolabelling

Today, a material science focuses on the nanoparticles synthesis in general and synthesizing them by biological entity in particular for their marvel production and its remarkable property. In this present study, synthesis of gold nanoparticles using photosynthetic microorganisms such as Coelastrella sp. (eukaryotes) and Phormidium sp. (prokaryotes) were reacted with Chloroauric acid (HAuCl4) and bioaccumulation was assessed. Various techniques were adopted for characterization of nanoparticles and compared. It was found to be 25 nm sized nanotriangles and 30 nm sized spherical shaped nanoparticles were synthesized by prokaryotic and eukaryotic microorganisms respectively by TEM analysis. Biogenic gold nanoparticles have potent antioxidant property and the interaction of gold nanoparticles with DNA was evaluated that biogenic nanoparticles were actively bound to DNA in increased concentration. It was revealed that biogenic nanoparticles have wide range of applications depends on the biological entity used. Selection of suitable biological entity is very much important for the production of nanoparticles with desirable shapes and size for the biomedical applications. (c) 2012 Elsevier B.V. All rights reserved.