期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 105, 期 -, 页码 230-235出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2012.12.055
关键词
Silver nanoparticles; Poly(N-vinyl-2-pyrrolidone); In vitro cytotoxicity; Silver release kinetics; Bioreactor conditions
资金
- Ministry of Education, Science and Technological Development, Republic of Serbia [III 45019, III 45005, OI 175011]
- International Atomic Energy Agency, Vienna [CRP: F23028, 15384]
Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. (C) 2013 Elsevier B.V. All rights reserved.
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